Bayer Leverkusen's top-four hopes suffered a blow after 2-1 defeat to Freiburg on home soil.
After the first half ended goalless, the visitors raced into the lead on 50 minutes through Ermedin Demirovic's fourth league goal of the season.
The lead was doubled 11 minutes later when Lucas Holer bagged his third of the campaign by firing past debutant Lucas Grill.
Leon Bailey did pull a goal back for Leverkusen on 70 minutes, but Freiburg were able to hold for three points on the day and move up to eighth spot while Leverkusen remain in sixth position.
Union Berlin and Hoffenheim could not be separated after playing to a 1-1 draw on Sunday afternoon.
Max Kruse netted the opener for the hosts from the penalty spot before an own goal from Nico Schlotterbeck levelled matters for Hoffenheim.
Augsburg extended their unbeaten run in the league to three matches with a 1-0 win away to Mainz.
Andre Hahn's 25th minute strike was enough to seal the deal for Augsburg, who find themselves in 13th position.
