Pep Guardiola got five players off his unwanted list, while Chelsea completed the most expensive transfer of Deadline Day - read all the latest updates in our transfer round-up.
Young Belgium defender Jason Denayer was the final departure of Guardiola on Deadline Day, following the likes of Joe Hart, Wilfried Bony, Samir Nasri and Eliaquim Mangala through the exit door - all five only completed loan deals though.
Meanwhile, Spurs completed the signing of France midfielder Moussa Sissoko in the final minutes of Wednesday. The midfielder became the most second expensive signing of the day, with Spurs sending £30 million to Newcastle for his services.
Chelsea completed the biggest transfer of Wednesday after brining David Luiz for a reported fee of £34 million back from Paris Saint-Germain.
Leicester City was one of 13 Premier League sides, who broke their club transfer record, after signing Algerian striker Islam Slimani from Sporting. Find all major confirmed deals in our list below.
Moussa Sissoko (Newcastle to Spurs)
David Luiz (PSG to Chelsea)
Jason Denayer (Man City to Sunderland
Lazar Markovic (Liverpool to Sporting Lisbon) loan
Hull City have continued with Deadline Day shopping and have brought in Dieumerci Mbokani on a season-long loan.
Enner Valencia has completed a season-long loan move to Ronald Koeman's Everton.
Islam Slimani (Sporting Lisbon to Leicester City)
West Bromwich Albion have signed Cameroon international, Allan Nyom from Watford.
Hull City have completed the signing of Manchester United midfielder, James Wier on a three-year deal.
Mario Balotelli (Liverpool to OGC Nice)
Tottenham Hotspur have completed the signing of Pau Lopez on a season-long loan from Espanyol.
David Luiz (Paris Saint-Germain to Chelsea)
Marseille winger, Georges-Kévin N'Koudou has joined Premier League outfit, Tottenham Hotspur.
Juan Cuadrado (Chelsea to Juventus) loan
Middlesbrough have confirmed the signing of former Barcelona winger, Adama Traore on a four-year deal.
Marcos Alonso (Fiorentina to Chelsea)
Eliaquim Mangala (Man City to Valencia) loan
Serge Gnabri (Arsenal to Werder Bremen) reported fee: €5 million
Samir Nasri (Man City to Sevilla) loan
Wilfried Bony (Man City to Stoke) loan
Joe Hart (Man City to Torino) loan
Burnley have completed the signing of Republic of Ireland midfielder, Jeff Hendrick for a club-record fee.
Stoke City have signed Netherlands international Bruno Martins Indi on a season-long loan from FC Porto.
Sunderland have broken their club transfer record to sign Gabon midfielder Didier Ndong for £13.6m from Lorient.
Tuesday's biggest transfers:
Paco Alcacer (Valencia – FC Barcelona) £25.5m
Gabriel Barbosa (Santos – Inter Milan) Undisclosed
Munir El Haddadi (Barcelona – Valencia) Loan
Tomas Rosicky (Arsenal – Sparta Prague) Free
Patrick Bamford (Chelsea – Burnley) Loan
Calum Chambers (Arsenal – Middlesbrough) Loan
Will Keane (Manchester United – Hull City) Undisclosed
Kenedy (Chelsea – Watford) Loan
Shkodran Mustafi (Valencia – Arsenal) About £35m
Lucas Perez (Deportivo La Coruna – Arsenal) £17.1m
Loic Remy (Chelsea – Crystal Palace) Loan
Yevhen Konoplyanka (Sevilla – Schalke) Loan
Note: We will update this Deadline Day Transfer Round-Up throughout the day to keep you informed about all the latest news.
Which players would you like to see your club sign today? Have your say in the comments section below.