OGC Nice striker Mario Balotelli was not included in Roberto Mancini's latest Italy squad, while a Paris Saint-Germain star has earned a recall.
Italy will host Ukraine in a friendly on 10 October, before they take on Poland in a Nations League clash in Chorzow on 16 October.
Balotelli was included in the last Italy squad in the previous international break, although Mancini was concerned about the striker's fitness.
After being dropped by Nice for Friday's game against Tolouse, Balotelli has now been left out by the Azzuri boss.
Meanwhile, PSG star Marco Verratti has earned a recall to the squad along with Major League Soccer star Sebastian Giovinco.
Goalkeepers: Alessio Cragno (Cagliari), Gianluigi Donnarumma (AC Milan), Mattia Perin (Juventus), Salvatore Sirigu (Torino)
Defenders: Francesco Acerbi (Lazio), Cristiano Biraghi (Fiorentina), Leonardo Bonucci (Juventus), Giorgio Chiellini (Juventus), Domenico Criscito (Genoa), Danilo D'Ambrosio (Inter Milan), Emerson Palmieri (Chelsea), Alessandro Florenzi (Roma), Alessio Romagnoli (AC Milan)
Midfielders: Nicolo Barella (Cagliari), Federico Bernardeschi (Juventus), Giacomo Bonaventura (AC Milan), Roberto Gagliardini (Inter Milan), Jorginho (Chelsea), Lorenzo Pellegrini (Roma), Marco Verratti (Paris Saint Germain)
Forwards: Domenico Berardi (Sassuolo), Gianluca Caprari (Sampdoria), Federico Chiesa (Fiorentina), Patrick Cutrone (AC Milan), Sebastian Giovinco (Toronto FC), Ciro Immobile (Lazio), Lorenzo Insigne (Napoli), Simone Zaza (Torino).