Serie A champions Juventus ended their season with a loss as they went down 3-2 to Sampdoria on Saturday night.
Citadin Eder cancelled out Fabio Quagliarella before strikes from Lorenzo De Silvestri and Mauro Icardi extended their advantage.
Emanuele Giaccherini pulled a goal back late on for the Old Lady but it was not enough as Samp completed a double of their opponents.
Juve looked to be on course to beat each team in the Serie A at least once this season when Andrea Pirlo pumped a superb pass into the path of Quagliarella who finished at the near post.
Their joy lasted just over five minutes though before Giorgio Chiellini brought down Icardi in the area and Eder slotted the resulting penalty.
De Silvestro then gave the hosts the perfect start to the second-half when he buried a bullet header past the goalkeeper.
Quagliarella should have made it 2-2 with 20 minutes to go but he shot straight at Junior Costa from six yards out.
That miss would be costly as five minutes later Icardi converted a cross from former Juve winger Marcelo Estigarribia.
Juve then seemed to earn a penalty when the referee pointed to the spot but he soon changed his mind after consulting with his assistant behind the goal.
The visitors refused to give in and Giaccherini found the top corner in stoppage time to give them some hope but there was not enough time for them to find an equaliser as Samp claimed the win.